Verification Tests of Antimicrobial Finish

The types of test for verification of antimicrobial finish in our research work is: Quantitative Test 

Qualitative Test helps us to verify that whether antimicrobial finish is applied properly on the substrate or not. The test that we commonly use on industrial scale is known as “BPB (Bromo Phenol Blue)” Test. In this research Quantitative test method has been followed Universal Beer Agar (UBA). 

Result and Discussion of Anti-Microbial Finish 

In this chapter, antimicrobial activity of the treated fabric is investigated against different concentration of Chitosan and turmeric 

Below here we listed the results of effectiveness of antimicrobial activity. 

Table 4.1: Results of effectiveness of antimicrobial activity

Table 4.2: Result of anti-microbial finish

e.g. Sample 1 to calculate the percentage reduction in standard time using 

% Reduction = [(A-B)/A] x 100 41-1]/41 X 100 =87.8% 

All the above sample are calculated by this methods 

Discussion of Testing Results of Antimicrobial Activity

It is concluded from testing results of antimicrobial activity that sample like 1 has 100% amount of turmeric in their recipes. Amount was less when we compare from the other samples (2, 3, 4&5). Even with in high washing time then we concluded that it comes in the category of Under Treated. 

Similarly when we compare Sample 2 from sample 1 then we concluded that it comes in the category of slightly significant. In this recipe, amount of Chitosan was 25% $ tumeric75%. 

When we again compare Sample 3 with like’s sample 1$ 2 in these recipes, amount of Chitosan was 50% $50% turmeric then we concluded that is more significant. 

Lastly when we camper sample 4$ 5there is no significant different between them both are more effective anti-microbial activity. 

Crease recovery angle: 

Crease recovery angle behaviour has been measured in terms of total crease recovery angle (CRA), which is the sum of crease recovery angel measured in warp and weft way CRA is only 77º for untreated fabric and for all treated the value range from 82 º to 110 which is comparable to that of treated control sample increase in the CRA is mainly due to cross linking of cellulose chine. 

As concentration of chitosan 100% treated samples show only a marginal increase in crease recovery angle values in the range 82º- 95º, it inferred the chitosan cannot improve crease recovery angel of cotton fabric significantly but, addition of turmeric helps to improve the crease recovery though the molecular cross liking and makes the fabric more functional. Almost all sample shows good creases recover angel around 110º (table) 

Table 4.3: Crease recovery angle

Bending length (C): 

The bending length is related to the angle that the fabric makes to the horizontal we calculate by using formula. when we compare Bending length (C) for untreated fabric 14.8cm and 100% chitosan we conclude that the fabric is stiffer, lack good drape, and lack flexibility. From treated one……. 

Table 4.4: Bending length

Table 4.5: Flexural rigidity

Conclusions 

The coating of cotton fabric using turmeric and chitosan natural product was found to exhibit antibacterial properties. It exhibits efficient antimicrobial agent for the preparation of antimicrobial finish of medical cloths. The antibacterial efficacy of only turmeric and 75% turmeric combination with 25% chitosan coated fabric was found to be less efficient compared to only chitosan and 25tumeric combination with 75% chitosan coated fabric may be due to their binding properties. The improvement in the anti microbial property may be due to present of cationised amino group (–NH+3) in the chitosan and not influence present of turmeric The present investigation highlighted that coated fabric shows antibacterial properties against gram negative bacteria and gram positive bacteria reports on the efficiency of indicating that this technique can be used in the textile industry as antimicrobial finish of medical cloths both for gram positive and gram negative bacteria as value added products.